Journal cover Journal topic
Atmospheric Measurement Techniques An interactive open-access journal of the European Geosciences Union
Journal topic

Journal metrics

Journal metrics

  • IF value: 3.248 IF 3.248
  • IF 5-year value: 3.650 IF 5-year 3.650
  • CiteScore value: 3.37 CiteScore 3.37
  • SNIP value: 1.253 SNIP 1.253
  • SJR value: 1.869 SJR 1.869
  • IPP value: 3.29 IPP 3.29
  • h5-index value: 47 h5-index 47
  • Scimago H index value: 60 Scimago H index 60
Volume 11, issue 10 | Copyright
Atmos. Meas. Tech., 11, 5767-5780, 2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Research article 19 Oct 2018

Research article | 19 Oct 2018

Development and field testing of an online instrument for measuring the real-time oxidative potential of ambient particulate matter based on dithiothreitol assay

Joseph V. Puthussery, Chen Zhang, and Vishal Verma Joseph V. Puthussery et al.
  • Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, 61801, USA

Abstract. We developed an online instrument for measuring the oxidative potential (OP) of ambient particulate matter (PM) using the dithiothreitol (DTT) assay. The instrument uses a mist chamber (MC) to continuously collect the ambient PM2.5 in water, and then determines its DTT activity using an automated syringe pump system. The instrument was deployed at an urban site in the University of Illinois campus, and its field performance was evaluated by comparing the results with the offline DTT activity measurements of simultaneously collected PM-laden filters. The online DTT activity measurements correlated well with the offline measurements but were higher than both methanol (slope = 1.08, R2 = 0.93) and Milli-Q water (slope = 1.86, R2 = 0.86) extracts of the PM filters, indicating a better efficiency of the MC for collecting the water-insoluble fraction of PM. The hourly measurements of ambient PM2.5 OP were obtained by running the online instrument intermittently for 50 days with minimal manual assistance. The daytime DTT activity levels were generally higher than at night. However, a 4-fold increase in the hourly averaged activity was observed on the night of 4 July (Independence Day fireworks display). The diurnal profile of the hourly averaged OP during weekdays showed a bimodal trend, with a sharp peak in the morning (around 07:00LT), followed by a broader afternoon peak which plateaus around 14:00LT and starts subsiding at night (around 19:00LT).

To investigate the association of the diurnal profile of DTT activity with the emission sources at the site, we collected time-segregated composite PM filter samples in four different time periods of the day (morning, 07:00–10:00LT; afternoon, 10:00–15:00LT; evening, 15:00–19:00LT; and night, 19:00–07:00LT) and determined the diurnal variations in the redox active components (i.e., water-soluble Cu, Fe, Mn, organic carbon, elemental carbon, and water-soluble organic carbon). Based on this comparison, we attributed the daytime OP of ambient PM2.5 to the vehicular (both exhaust and non-exhaust) emissions and resuspended dust, whereas secondary photochemical transformation of primary emissions appear to enhance the OP of PM during the afternoon and evening period.

Publications Copernicus
Short summary
The oxidative potential (OP) of ambient particulate matter (PM) has recently gained attention as an alternative metric for assessing the ambient PM toxicity. However, a major constraint in measuring the OP is its labor-intensive protocol. Here, we developed a new online instrument by coupling a mist chamber to an automated analytical system which can measure the real-time OP of ambient PM. We also report for the first time, the hourly averaged diurnal profile of ambient PM OP at an urban site.
The oxidative potential (OP) of ambient particulate matter (PM) has recently gained attention as...