Twin-plate Ice Nucleation Assay (TINA) with infrared detection for high-throughput droplet freezing experiments with biological ice nuclei in laboratory and field samples

Kunert, Anna T.; Lamneck, Mark; Helleis, Frank; Pöschl, Ulrich; Pöhlker, Mira L.; Fröhlich-Nowoisky, Janine

doi:https://doi.org/10.5194/amt-11-6327-2018

Articles | Volume 11, issue 11

https://doi.org/10.5194/amt-11-6327-2018

© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Special issue:

Results from the ice nucleation research unit (INUIT) (ACP/AMT...

https://doi.org/10.5194/amt-11-6327-2018

© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 11, issue 11

Research article

|

23 Nov 2018

Research article |

| 23 Nov 2018

Twin-plate Ice Nucleation Assay (TINA) with infrared detection for high-throughput droplet freezing experiments with biological ice nuclei in laboratory and field samples

Anna T. Kunert, Mark Lamneck, Frank Helleis, Ulrich Pöschl, Mira L. Pöhlker, and Janine Fröhlich-Nowoisky

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Final revised paper (published on 23 Nov 2018)
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Preprint (discussion started on 24 Jul 2018)
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Interactive discussion

Status: closed

AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

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RC1: 'Review of amt-2018-230 by Kunert et al.', Anonymous Referee #1, 30 Aug 2018
- AC1: 'Response to Interactive Comment by Anonymous Referee #1', Anna Theresa Kunert, 07 Nov 2018
RC2: 'Review for the manuscript by Kunert et al.', Anonymous Referee #2, 02 Sep 2018
- AC2: 'Response to Interactive Comment by Anonymous Referee #2', Anna Theresa Kunert, 07 Nov 2018

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision

AR by Anna Theresa Kunert on behalf of the Authors (07 Nov 2018) Author's response Manuscript

ED: Publish as is (11 Nov 2018) by Mingjin Tang

Short summary

The new Twin-plate Ice Nucleation Assay with infrared detection for high-throughput droplet freezing experiments in microliter-sized droplets is introduced, which was tested and characterized with bacterial and fungal ice nuclei. It was applied to investigate the influence of chemical processing on the activity of biological ice nuclei, and aqueous extracts of atmospheric aerosols were studied for ice nuclei activity.