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Volume 8, issue 1
Atmos. Meas. Tech., 8, 471–482, 2015
https://doi.org/10.5194/amt-8-471-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.
Atmos. Meas. Tech., 8, 471–482, 2015
https://doi.org/10.5194/amt-8-471-2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.

Research article 29 Jan 2015

Research article | 29 Jan 2015

A semi-automated system for quantifying the oxidative potential of ambient particles in aqueous extracts using the dithiothreitol (DTT) assay: results from the Southeastern Center for Air Pollution and Epidemiology (SCAPE)

T. Fang et al.

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Cited articles

Alfadda, A. A. and Sallam, R. M.: Reactive oxygen species in health and disease, J. Biomed. Biotechnol., 2012, 936486, https://doi.org/10.1155/2012/936486, 2012.
Antonini, J. M., Clarke, R. W., Krishna Murthy, G. G., Sreekanthan, P., Jenkins, N., Eagar, T. W., and Brain, J. D.: Freshly generated stainless steel welding fume induces greater lung inflammation in rats as compared to aged fume, Toxicol. Lett., 98, 77–86, 1998.
Aust, A. E., Ball, J. C., Hu, A. A., Lighty, J. S., Smith, K. R., Straccia, A. M., Veranth, J. M., and Young, W. C.: Particle characteristics responsible for effects on human lung epithelial cells, Res. Rep. Health Eff. Inst., 110, 1–65, 2002.
Bonvallot, V., Baeza-Squiban, A., Baulig, A., Brulant, S., Boland, S., Muzeau, F., Barouki, R., and Marano, F.: Organic compounds from diesel exhaust particles elicit a proinflammatory response in human airway epithelial cells and induce cytochrome p450 1A1 expression, Am. J. Resp. Cell Mol., 25, 515–521, 2001.
Charrier, J. G. and Anastasio, C.: On dithiothreitol (DTT) as a measure of oxidative potential for ambient particles: evidence for the importance of soluble transition metals, Atmos. Chem. Phys., 12, 9321–9333, https://doi.org/10.5194/acp-12-9321-2012, 2012.
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This work summarizes a newly developed semi-automated system for quantifying the oxidative potential of aerosol aqueous extracts using the dithiothreitol (DTT) assay. 500 sample analyses indicate that DTT activity in the southeast US is likely not dominated by a unique local source, and sources change with season. The unique large data set generated with the technique described in this paper allows new studies on DTT sources and investigating linkages between reactive oxygen species and health.
This work summarizes a newly developed semi-automated system for quantifying the oxidative...
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